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It uses Hotstart Taq DNA polymerase and a specially formulated buffer system
It can glucosylate 5-hmC on 1 μg of DNA in 15 minutes at 37°C
but it is inactive for single-stranded nucleic acids
The 4X RT-ddPCR Master Mix is a master mix specifically designed for one-step multiplex digital fluorescent quantitative PCR
The product of Hotstart Taq has overhanged dA at 3'-end
GDSlyo Taq DNA Polymerase Spec.:P1261/500 U It uses Hotstart Taq DNAGDSlyo Taq DNA Polymerase is a Taq DNA Polymerase intended for the preparation of freeze dried reagents, also known as the lyophilizable version of Taq DNA Polymerase. It is derived from the thermophilic bacterium Thermus aquaticus and has a molecular weight of 94 kD. Taq DNA Polymerase can amplify DNA target up to 10 kb (simple template). The elongation velocity is 2kb min (70~75C). It has 5' to 3' polymerase activity but lacks of 3' to 5'